It is not exported by DAPAR
and has to be reproduced here.
Value
A list of two dataframes : logFC and P_Value. The first one contains the logFC values of all the comparisons (one column for one comparison), the second one contains the pvalue of all the comparisons (one column for one comparison). The names of the columns for those two dataframes are identical and correspond to the description of the comparison.
Author
Adapted from the code of Samuel Wieczorek in the DAPAR
package as it is an object that is not exported by the DAPAR
package.
Examples
# library(DAPAR)
set.seed(2016)
data(qData)
data(sTab)
contrast=1
sTab.old <- sTab
conds <- factor(sTab$Condition, levels = unique(sTab$Condition))
sTab <- sTab[unlist(lapply(split(sTab, conds), function(x) {
x["Sample.name"]
})), ]
qData <- qData[, unlist(lapply(split(sTab.old, conds), function(x) {
x["Sample.name"]
}))]
conds <- conds[order(conds)]
res.l <- NULL
design.matrix <- mi4p::make.design(sTab)
contra <- mi4p::make.contrast(design.matrix, condition = conds,
contrast)
cmtx <- limma::makeContrasts(contrasts = contra, levels = make.names(colnames(design.matrix)))
fit <- limma::eBayes(limma::contrasts.fit(limma::lmFit(qData,
design.matrix), cmtx))
#> Warning: Partial NA coefficients for 32 probe(s)
res.l <- mi4p::formatLimmaResult(fit, conds, contrast)